Yes. Any conference call can have the host plus up to 25 participants, and these can be either people using Skype or calling in on a landline or mobile.
However, if you are using a version of Skype for Windows older than 3.8, you can only host up to 4 participants.
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Each call will be charged separately and each call will appear on your account as a separate call. If you have less than 1 minute of Skype Credit remaining during the conference call, then this call will terminate unless you have a means of adding more Skype Credit when requested. Other calls will continue until they have less than one minute of Skype Credit left.
There is no additional charge for calling landlines or mobiles during a conference call - you simply pay for the time used, for each call.